prdm16 mouse reverse Search Results


gene exp dio2 mm00515664 m1  (Thermo Fisher)
92
Thermo Fisher gene exp dio2 mm00515664 m1
Gene Exp Dio2 Mm00515664 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prdm16+mouse+reverse/pm33053350-220-57-61?v=Thermo+Fisher
Average 92 stars, based on 1 article reviews
gene exp dio2 mm00515664 m1 - by Bioz Stars, 2026-07
92/100 stars
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prdm16 mouse reverse  (Eurofins)
86
Eurofins prdm16 mouse reverse
Prdm16 Mouse Reverse, supplied by Eurofins, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prdm16+mouse+reverse/pmc13016079-133-0-4?v=Eurofins
Average 86 stars, based on 1 article reviews
prdm16 mouse reverse - by Bioz Stars, 2026-07
86/100 stars
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129 tg  (Jackson Laboratory)
86
Jackson Laboratory 129 tg
129 Tg, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prdm16+mouse+reverse/pm32610084-272-0-5?v=Jackson+Laboratory
Average 86 stars, based on 1 article reviews
129 tg - by Bioz Stars, 2026-07
86/100 stars
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rneasy plus mini kit  (Qiagen)
99
Qiagen rneasy plus mini kit
Rneasy Plus Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prdm16+mouse+reverse/pmc09800552-323-6-10?v=Qiagen
Average 99 stars, based on 1 article reviews
rneasy plus mini kit - by Bioz Stars, 2026-07
99/100 stars
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prism 8.0  (GraphPad Software Inc)
90
GraphPad Software Inc prism 8.0
Prism 8.0, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prdm16+mouse+reverse/pmc07384974-965-128-131?v=GraphPad+Software+Inc
Average 90 stars, based on 1 article reviews
prism 8.0 - by Bioz Stars, 2026-07
90/100 stars
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assays digoxigenin dutp labeling kit roche n a in situ hybridization chain reaction v3 0 hcr  (Molecular Instruments)
86
Molecular Instruments assays digoxigenin dutp labeling kit roche n a in situ hybridization chain reaction v3 0 hcr
Assays Digoxigenin Dutp Labeling Kit Roche N A In Situ Hybridization Chain Reaction V3 0 Hcr, supplied by Molecular Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prdm16+mouse+reverse/pm37676768-240-88-101?v=Molecular+Instruments
Average 86 stars, based on 1 article reviews
assays digoxigenin dutp labeling kit roche n a in situ hybridization chain reaction v3 0 hcr - by Bioz Stars, 2026-07
86/100 stars
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axs-1114544  (Axis-Shield Diagnostics)
90
Axis-Shield Diagnostics axs-1114544
Axs 1114544, supplied by Axis-Shield Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prdm16+mouse+reverse/pm38963763-226-60-61?v=Axis-Shield+Diagnostics
Average 90 stars, based on 1 article reviews
axs-1114544 - by Bioz Stars, 2026-07
90/100 stars
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trizol reagent invitrogen  (Thermo Fisher)
99
Thermo Fisher trizol reagent invitrogen
Trizol Reagent Invitrogen, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prdm16+mouse+reverse/pm32160547-162-104-106?v=Thermo+Fisher
Average 99 stars, based on 1 article reviews
trizol reagent invitrogen - by Bioz Stars, 2026-07
99/100 stars
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ctbp1  (Proteintech)
93
Proteintech ctbp1
Primer sequences used for RT-qPCR experiments.
Ctbp1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prdm16+mouse+reverse/pmc11329929-137-7-37?v=Proteintech
Average 93 stars, based on 1 article reviews
ctbp1 - by Bioz Stars, 2026-07
93/100 stars
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pgc 1α  (Proteintech)
96
Proteintech pgc 1α
Primer sequences used for RT-qPCR experiments.
Pgc 1α, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prdm16+mouse+reverse/pmc11329929-137-4-37?v=Proteintech
Average 96 stars, based on 1 article reviews
pgc 1α - by Bioz Stars, 2026-07
96/100 stars
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pparγ  (Proteintech)
96
Proteintech pparγ
Primer sequences used for RT-qPCR experiments.
Pparγ, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prdm16+mouse+reverse/pmc11329929-137-6-37?v=Proteintech
Average 96 stars, based on 1 article reviews
pparγ - by Bioz Stars, 2026-07
96/100 stars
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pparα  (Proteintech)
96
Proteintech pparα
Primer sequences used for RT-qPCR experiments.
Pparα, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prdm16+mouse+reverse/pmc11329929-137-5-37?v=Proteintech
Average 96 stars, based on 1 article reviews
pparα - by Bioz Stars, 2026-07
96/100 stars
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Image Search Results


Primer sequences used for RT-qPCR experiments.

Journal: Frontiers in Physiology

Article Title: Ling-gui-zhu-gan granules reduces obesity and ameliorates metabolic disorders by inducing white adipose tissue browning in obese mice

doi: 10.3389/fphys.2024.1427722

Figure Lengend Snippet: Primer sequences used for RT-qPCR experiments.

Article Snippet: Primary antibodies to UCP1, PGC-1α, PPARα, PPARγ, CTBP1, CTBP2, and β-Tubulin (Cat no: 23673-1-AP, 66369-1-Ig, 15540-1-AP, 16643-1-AP, 10972-1-AP, 10346-1-AP, and 66240-1-lg) and secondary antibodies to rabbit and mouse (Cat no: SA00001-1 and SA00001-2) were purchased from the Proteintech Group (Chicago, IL, United States), while the primary antibody to PRDM16 (Cat no: PA5-20872) was from Invitrogen (Carlsbad, CA, United States).

Techniques:

Effect of LGZG on the expression of thermogenesis-related genes and proteins in HFD-induced obese mice. (A) Relative genes expressions of UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in eWAT. (B,C) The image analysis and quantitative analysis results of proteins to UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in eWAT. (D) Relative genes expressions of UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in sWAT. (E,F) The image analysis and quantitative analysis results of proteins to UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in sWAT. All data were presented as mean ± SEM, with n = 3∼6 in (A) , n = 5 in (D) , n = 3 in (B,C,E,F) . # p < 0.05 vs. the NC group, * p < 0.05 vs. the OB group. UCP1, uncoupling protein 1; PRDM16, PR domain containing 16; PGC-1α, peroxisome proliferator-activated receptor γ coactivator-1α; PPARα, peroxisome proliferator activated receptor α; PPARγ, peroxisome proliferator activated receptor γ; CTBP1, C-terminal binding proteins 1; CTBP2, C-terminal binding proteins 2; eWAT, epididymal white adipose tissue; sWAT, subcutaneous white adipose tissue.

Journal: Frontiers in Physiology

Article Title: Ling-gui-zhu-gan granules reduces obesity and ameliorates metabolic disorders by inducing white adipose tissue browning in obese mice

doi: 10.3389/fphys.2024.1427722

Figure Lengend Snippet: Effect of LGZG on the expression of thermogenesis-related genes and proteins in HFD-induced obese mice. (A) Relative genes expressions of UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in eWAT. (B,C) The image analysis and quantitative analysis results of proteins to UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in eWAT. (D) Relative genes expressions of UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in sWAT. (E,F) The image analysis and quantitative analysis results of proteins to UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in sWAT. All data were presented as mean ± SEM, with n = 3∼6 in (A) , n = 5 in (D) , n = 3 in (B,C,E,F) . # p < 0.05 vs. the NC group, * p < 0.05 vs. the OB group. UCP1, uncoupling protein 1; PRDM16, PR domain containing 16; PGC-1α, peroxisome proliferator-activated receptor γ coactivator-1α; PPARα, peroxisome proliferator activated receptor α; PPARγ, peroxisome proliferator activated receptor γ; CTBP1, C-terminal binding proteins 1; CTBP2, C-terminal binding proteins 2; eWAT, epididymal white adipose tissue; sWAT, subcutaneous white adipose tissue.

Article Snippet: Primary antibodies to UCP1, PGC-1α, PPARα, PPARγ, CTBP1, CTBP2, and β-Tubulin (Cat no: 23673-1-AP, 66369-1-Ig, 15540-1-AP, 16643-1-AP, 10972-1-AP, 10346-1-AP, and 66240-1-lg) and secondary antibodies to rabbit and mouse (Cat no: SA00001-1 and SA00001-2) were purchased from the Proteintech Group (Chicago, IL, United States), while the primary antibody to PRDM16 (Cat no: PA5-20872) was from Invitrogen (Carlsbad, CA, United States).

Techniques: Expressing, Binding Assay

Journal: Frontiers in Physiology

Article Title: Ling-gui-zhu-gan granules reduces obesity and ameliorates metabolic disorders by inducing white adipose tissue browning in obese mice

doi: 10.3389/fphys.2024.1427722

Figure Lengend Snippet:

Article Snippet: Primary antibodies to UCP1, PGC-1α, PPARα, PPARγ, CTBP1, CTBP2, and β-Tubulin (Cat no: 23673-1-AP, 66369-1-Ig, 15540-1-AP, 16643-1-AP, 10972-1-AP, 10346-1-AP, and 66240-1-lg) and secondary antibodies to rabbit and mouse (Cat no: SA00001-1 and SA00001-2) were purchased from the Proteintech Group (Chicago, IL, United States), while the primary antibody to PRDM16 (Cat no: PA5-20872) was from Invitrogen (Carlsbad, CA, United States).

Techniques: Binding Assay, High Performance Liquid Chromatography, Control, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot

Primer sequences used for RT-qPCR experiments.

Journal: Frontiers in Physiology

Article Title: Ling-gui-zhu-gan granules reduces obesity and ameliorates metabolic disorders by inducing white adipose tissue browning in obese mice

doi: 10.3389/fphys.2024.1427722

Figure Lengend Snippet: Primer sequences used for RT-qPCR experiments.

Article Snippet: Primary antibodies to UCP1, PGC-1α, PPARα, PPARγ, CTBP1, CTBP2, and β-Tubulin (Cat no: 23673-1-AP, 66369-1-Ig, 15540-1-AP, 16643-1-AP, 10972-1-AP, 10346-1-AP, and 66240-1-lg) and secondary antibodies to rabbit and mouse (Cat no: SA00001-1 and SA00001-2) were purchased from the Proteintech Group (Chicago, IL, United States), while the primary antibody to PRDM16 (Cat no: PA5-20872) was from Invitrogen (Carlsbad, CA, United States).

Techniques:

Effect of LGZG on the expression of thermogenesis-related genes and proteins in HFD-induced obese mice. (A) Relative genes expressions of UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in eWAT. (B,C) The image analysis and quantitative analysis results of proteins to UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in eWAT. (D) Relative genes expressions of UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in sWAT. (E,F) The image analysis and quantitative analysis results of proteins to UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in sWAT. All data were presented as mean ± SEM, with n = 3∼6 in (A) , n = 5 in (D) , n = 3 in (B,C,E,F) . # p < 0.05 vs. the NC group, * p < 0.05 vs. the OB group. UCP1, uncoupling protein 1; PRDM16, PR domain containing 16; PGC-1α, peroxisome proliferator-activated receptor γ coactivator-1α; PPARα, peroxisome proliferator activated receptor α; PPARγ, peroxisome proliferator activated receptor γ; CTBP1, C-terminal binding proteins 1; CTBP2, C-terminal binding proteins 2; eWAT, epididymal white adipose tissue; sWAT, subcutaneous white adipose tissue.

Journal: Frontiers in Physiology

Article Title: Ling-gui-zhu-gan granules reduces obesity and ameliorates metabolic disorders by inducing white adipose tissue browning in obese mice

doi: 10.3389/fphys.2024.1427722

Figure Lengend Snippet: Effect of LGZG on the expression of thermogenesis-related genes and proteins in HFD-induced obese mice. (A) Relative genes expressions of UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in eWAT. (B,C) The image analysis and quantitative analysis results of proteins to UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in eWAT. (D) Relative genes expressions of UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in sWAT. (E,F) The image analysis and quantitative analysis results of proteins to UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in sWAT. All data were presented as mean ± SEM, with n = 3∼6 in (A) , n = 5 in (D) , n = 3 in (B,C,E,F) . # p < 0.05 vs. the NC group, * p < 0.05 vs. the OB group. UCP1, uncoupling protein 1; PRDM16, PR domain containing 16; PGC-1α, peroxisome proliferator-activated receptor γ coactivator-1α; PPARα, peroxisome proliferator activated receptor α; PPARγ, peroxisome proliferator activated receptor γ; CTBP1, C-terminal binding proteins 1; CTBP2, C-terminal binding proteins 2; eWAT, epididymal white adipose tissue; sWAT, subcutaneous white adipose tissue.

Article Snippet: Primary antibodies to UCP1, PGC-1α, PPARα, PPARγ, CTBP1, CTBP2, and β-Tubulin (Cat no: 23673-1-AP, 66369-1-Ig, 15540-1-AP, 16643-1-AP, 10972-1-AP, 10346-1-AP, and 66240-1-lg) and secondary antibodies to rabbit and mouse (Cat no: SA00001-1 and SA00001-2) were purchased from the Proteintech Group (Chicago, IL, United States), while the primary antibody to PRDM16 (Cat no: PA5-20872) was from Invitrogen (Carlsbad, CA, United States).

Techniques: Expressing, Binding Assay

Journal: Frontiers in Physiology

Article Title: Ling-gui-zhu-gan granules reduces obesity and ameliorates metabolic disorders by inducing white adipose tissue browning in obese mice

doi: 10.3389/fphys.2024.1427722

Figure Lengend Snippet:

Article Snippet: Primary antibodies to UCP1, PGC-1α, PPARα, PPARγ, CTBP1, CTBP2, and β-Tubulin (Cat no: 23673-1-AP, 66369-1-Ig, 15540-1-AP, 16643-1-AP, 10972-1-AP, 10346-1-AP, and 66240-1-lg) and secondary antibodies to rabbit and mouse (Cat no: SA00001-1 and SA00001-2) were purchased from the Proteintech Group (Chicago, IL, United States), while the primary antibody to PRDM16 (Cat no: PA5-20872) was from Invitrogen (Carlsbad, CA, United States).

Techniques: Binding Assay, High Performance Liquid Chromatography, Control, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot

Primer sequences used for RT-qPCR experiments.

Journal: Frontiers in Physiology

Article Title: Ling-gui-zhu-gan granules reduces obesity and ameliorates metabolic disorders by inducing white adipose tissue browning in obese mice

doi: 10.3389/fphys.2024.1427722

Figure Lengend Snippet: Primer sequences used for RT-qPCR experiments.

Article Snippet: Primary antibodies to UCP1, PGC-1α, PPARα, PPARγ, CTBP1, CTBP2, and β-Tubulin (Cat no: 23673-1-AP, 66369-1-Ig, 15540-1-AP, 16643-1-AP, 10972-1-AP, 10346-1-AP, and 66240-1-lg) and secondary antibodies to rabbit and mouse (Cat no: SA00001-1 and SA00001-2) were purchased from the Proteintech Group (Chicago, IL, United States), while the primary antibody to PRDM16 (Cat no: PA5-20872) was from Invitrogen (Carlsbad, CA, United States).

Techniques:

Effect of LGZG on the expression of thermogenesis-related genes and proteins in HFD-induced obese mice. (A) Relative genes expressions of UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in eWAT. (B,C) The image analysis and quantitative analysis results of proteins to UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in eWAT. (D) Relative genes expressions of UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in sWAT. (E,F) The image analysis and quantitative analysis results of proteins to UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in sWAT. All data were presented as mean ± SEM, with n = 3∼6 in (A) , n = 5 in (D) , n = 3 in (B,C,E,F) . # p < 0.05 vs. the NC group, * p < 0.05 vs. the OB group. UCP1, uncoupling protein 1; PRDM16, PR domain containing 16; PGC-1α, peroxisome proliferator-activated receptor γ coactivator-1α; PPARα, peroxisome proliferator activated receptor α; PPARγ, peroxisome proliferator activated receptor γ; CTBP1, C-terminal binding proteins 1; CTBP2, C-terminal binding proteins 2; eWAT, epididymal white adipose tissue; sWAT, subcutaneous white adipose tissue.

Journal: Frontiers in Physiology

Article Title: Ling-gui-zhu-gan granules reduces obesity and ameliorates metabolic disorders by inducing white adipose tissue browning in obese mice

doi: 10.3389/fphys.2024.1427722

Figure Lengend Snippet: Effect of LGZG on the expression of thermogenesis-related genes and proteins in HFD-induced obese mice. (A) Relative genes expressions of UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in eWAT. (B,C) The image analysis and quantitative analysis results of proteins to UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in eWAT. (D) Relative genes expressions of UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in sWAT. (E,F) The image analysis and quantitative analysis results of proteins to UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in sWAT. All data were presented as mean ± SEM, with n = 3∼6 in (A) , n = 5 in (D) , n = 3 in (B,C,E,F) . # p < 0.05 vs. the NC group, * p < 0.05 vs. the OB group. UCP1, uncoupling protein 1; PRDM16, PR domain containing 16; PGC-1α, peroxisome proliferator-activated receptor γ coactivator-1α; PPARα, peroxisome proliferator activated receptor α; PPARγ, peroxisome proliferator activated receptor γ; CTBP1, C-terminal binding proteins 1; CTBP2, C-terminal binding proteins 2; eWAT, epididymal white adipose tissue; sWAT, subcutaneous white adipose tissue.

Article Snippet: Primary antibodies to UCP1, PGC-1α, PPARα, PPARγ, CTBP1, CTBP2, and β-Tubulin (Cat no: 23673-1-AP, 66369-1-Ig, 15540-1-AP, 16643-1-AP, 10972-1-AP, 10346-1-AP, and 66240-1-lg) and secondary antibodies to rabbit and mouse (Cat no: SA00001-1 and SA00001-2) were purchased from the Proteintech Group (Chicago, IL, United States), while the primary antibody to PRDM16 (Cat no: PA5-20872) was from Invitrogen (Carlsbad, CA, United States).

Techniques: Expressing, Binding Assay

Journal: Frontiers in Physiology

Article Title: Ling-gui-zhu-gan granules reduces obesity and ameliorates metabolic disorders by inducing white adipose tissue browning in obese mice

doi: 10.3389/fphys.2024.1427722

Figure Lengend Snippet:

Article Snippet: Primary antibodies to UCP1, PGC-1α, PPARα, PPARγ, CTBP1, CTBP2, and β-Tubulin (Cat no: 23673-1-AP, 66369-1-Ig, 15540-1-AP, 16643-1-AP, 10972-1-AP, 10346-1-AP, and 66240-1-lg) and secondary antibodies to rabbit and mouse (Cat no: SA00001-1 and SA00001-2) were purchased from the Proteintech Group (Chicago, IL, United States), while the primary antibody to PRDM16 (Cat no: PA5-20872) was from Invitrogen (Carlsbad, CA, United States).

Techniques: Binding Assay, High Performance Liquid Chromatography, Control, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot

Primer sequences used for RT-qPCR experiments.

Journal: Frontiers in Physiology

Article Title: Ling-gui-zhu-gan granules reduces obesity and ameliorates metabolic disorders by inducing white adipose tissue browning in obese mice

doi: 10.3389/fphys.2024.1427722

Figure Lengend Snippet: Primer sequences used for RT-qPCR experiments.

Article Snippet: Primary antibodies to UCP1, PGC-1α, PPARα, PPARγ, CTBP1, CTBP2, and β-Tubulin (Cat no: 23673-1-AP, 66369-1-Ig, 15540-1-AP, 16643-1-AP, 10972-1-AP, 10346-1-AP, and 66240-1-lg) and secondary antibodies to rabbit and mouse (Cat no: SA00001-1 and SA00001-2) were purchased from the Proteintech Group (Chicago, IL, United States), while the primary antibody to PRDM16 (Cat no: PA5-20872) was from Invitrogen (Carlsbad, CA, United States).

Techniques:

Effect of LGZG on the expression of thermogenesis-related genes and proteins in HFD-induced obese mice. (A) Relative genes expressions of UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in eWAT. (B,C) The image analysis and quantitative analysis results of proteins to UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in eWAT. (D) Relative genes expressions of UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in sWAT. (E,F) The image analysis and quantitative analysis results of proteins to UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in sWAT. All data were presented as mean ± SEM, with n = 3∼6 in (A) , n = 5 in (D) , n = 3 in (B,C,E,F) . # p < 0.05 vs. the NC group, * p < 0.05 vs. the OB group. UCP1, uncoupling protein 1; PRDM16, PR domain containing 16; PGC-1α, peroxisome proliferator-activated receptor γ coactivator-1α; PPARα, peroxisome proliferator activated receptor α; PPARγ, peroxisome proliferator activated receptor γ; CTBP1, C-terminal binding proteins 1; CTBP2, C-terminal binding proteins 2; eWAT, epididymal white adipose tissue; sWAT, subcutaneous white adipose tissue.

Journal: Frontiers in Physiology

Article Title: Ling-gui-zhu-gan granules reduces obesity and ameliorates metabolic disorders by inducing white adipose tissue browning in obese mice

doi: 10.3389/fphys.2024.1427722

Figure Lengend Snippet: Effect of LGZG on the expression of thermogenesis-related genes and proteins in HFD-induced obese mice. (A) Relative genes expressions of UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in eWAT. (B,C) The image analysis and quantitative analysis results of proteins to UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in eWAT. (D) Relative genes expressions of UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in sWAT. (E,F) The image analysis and quantitative analysis results of proteins to UCP1, PRDM16, PGC-1α, PPARα, PPARγ, CTBP1, and CTBP2 in sWAT. All data were presented as mean ± SEM, with n = 3∼6 in (A) , n = 5 in (D) , n = 3 in (B,C,E,F) . # p < 0.05 vs. the NC group, * p < 0.05 vs. the OB group. UCP1, uncoupling protein 1; PRDM16, PR domain containing 16; PGC-1α, peroxisome proliferator-activated receptor γ coactivator-1α; PPARα, peroxisome proliferator activated receptor α; PPARγ, peroxisome proliferator activated receptor γ; CTBP1, C-terminal binding proteins 1; CTBP2, C-terminal binding proteins 2; eWAT, epididymal white adipose tissue; sWAT, subcutaneous white adipose tissue.

Article Snippet: Primary antibodies to UCP1, PGC-1α, PPARα, PPARγ, CTBP1, CTBP2, and β-Tubulin (Cat no: 23673-1-AP, 66369-1-Ig, 15540-1-AP, 16643-1-AP, 10972-1-AP, 10346-1-AP, and 66240-1-lg) and secondary antibodies to rabbit and mouse (Cat no: SA00001-1 and SA00001-2) were purchased from the Proteintech Group (Chicago, IL, United States), while the primary antibody to PRDM16 (Cat no: PA5-20872) was from Invitrogen (Carlsbad, CA, United States).

Techniques: Expressing, Binding Assay

Journal: Frontiers in Physiology

Article Title: Ling-gui-zhu-gan granules reduces obesity and ameliorates metabolic disorders by inducing white adipose tissue browning in obese mice

doi: 10.3389/fphys.2024.1427722

Figure Lengend Snippet:

Article Snippet: Primary antibodies to UCP1, PGC-1α, PPARα, PPARγ, CTBP1, CTBP2, and β-Tubulin (Cat no: 23673-1-AP, 66369-1-Ig, 15540-1-AP, 16643-1-AP, 10972-1-AP, 10346-1-AP, and 66240-1-lg) and secondary antibodies to rabbit and mouse (Cat no: SA00001-1 and SA00001-2) were purchased from the Proteintech Group (Chicago, IL, United States), while the primary antibody to PRDM16 (Cat no: PA5-20872) was from Invitrogen (Carlsbad, CA, United States).

Techniques: Binding Assay, High Performance Liquid Chromatography, Control, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot